Table 1.

Comparison of wild-type and mutant virus replication

MOI and mutant virus	Viral titera
	0 hpi	24 hpi	48 hpi
MOI, 0.2
Wild type	6.00 × 101 ± 28.3	1.67 × 107 ± 9.02 × 105	1.63 × 107 ± 6.11 × 105
ΔgE	4.00 × 101 ± 0.00	8.67 × 106 ± 1.16 × 106	2.31 × 107 ± 1.35 × 106
gDΔct	2.00 × 101 ± 0.00	2.88 × 106 ± 1.68 × 105	1.56 × 107 ± 8.72 × 105
gDΔct-ΔgE	3.00 × 101 ± 14.1	5.67 × 105 ± 4.37 × 104	6.53 × 106 ± 1.12 × 106
ΔgM2	2.00 × 101 ± 0.00	4.27 × 106 ± 7.69 × 105	4.80 × 106 ± 6.11 × 105
ΔgM2-ΔgE	1.00 × 101 ± 14.1	4.07 × 106 ± 5.21 × 105	9.20 × 106 ± 5.03 × 105
gDΔct-ΔgM2-ΔgE	3.00 × 101 ± 14.1	5.73 × 105 ± 5.93 × 104	2.55 × 106 ± 1.55 × 105
ΔUL11	1.00 × 101 ± 14.1	4.53 × 105 ± 4.16 × 104	1.14 × 107 ± 1.44 × 106
ΔUL11-ΔgM2	2.00 × 101 ± 0.00	1.13 × 105 ± 1.62 × 104	1.64 × 106 ± 8.00 × 104
ΔUL20	4.00 × 101 ± 28.3	3.81 × 104 ± 6.22 × 102	6.13 × 104 ± 2.31 × 103
MOI, 3.0
Wild type	4.60 × 102 ± 84.8	1.03 × 107 ± 8.97 × 105	7.61 × 106 ± 2.39 × 106
ΔgE	2.50 × 102 ± 42.4	1.27 × 107 ± 1.43 × 106	8.55 × 106 ± 3.76 × 106
gDΔct	2.40 × 102 ± 28.3	3.99 × 106 ± 3.15 × 105	4.40 × 106 ± 5.77 × 105
gDΔct-ΔgE	1.80 × 102 ± 56.6	3.40 × 105 ± 2.31 × 104	3.60 × 106 ± 6.43 × 105
ΔgM2	1.60 × 102 ± 56.6	4.73 × 106 ± 5.21 × 105	4.73 × 106 ± 7.33 × 105
ΔgM2-ΔgE	7.00 × 101 ± 14.1	7.13 × 106 ± 1.10 × 106	7.13 × 106 ± 8.51 × 105
gDΔct-ΔgM2-ΔgE	1.50 × 102 ± 14.1	3.17 × 106 ± 1.89 × 105	2.43 × 106 ± 1.30 × 105
ΔUL11	3.80 × 102 ± 28.3	1.99 × 107 ± 4.81 × 105	2.41 × 107 ± 1.33 × 105
ΔUL11-ΔgM2	ND	ND	ND
ΔUL20	2.70 × 102 ± 14.1	1.34 × 105 ± 2.34 × 103	1.61 × 105 ± 3.51 × 103

^aViral titers were determined at different times after infection of Vero cells at an MOI of 0.2 or an MOI of 3.0. The experiment was performed a second time, and the titers obtained were averaged, with the standard deviation calculated for each time point. ND, not done.