Table 2.
Determination of viral particle-to-PFU ratios
| Virus | No. of particles/PFU |
Overall defecta | |
|---|---|---|---|
| Cytoplasm | Supernatant | ||
| Wild type | 109 | 6 | + |
| ΔgE | 425 | 4 | ++ |
| gDΔct | 381 | 10 | ++ |
| gDΔct-ΔgE | 855 | 218 | +++ |
| ΔgM2 | 2,355 | 4 | ++ |
| ΔgM2-ΔgE | 417 | 9 | ++ |
| gDΔct-ΔgM2-ΔgE | 1,779 | 12 | +++ |
| ΔUL11 | 590 | 60 | +++ |
| ΔUL11-ΔgM2 | 3,460 | 70 | ++++ |
| ΔUL20 | 8,509 | 1,702b | +++++ |
a
Takes into account data from Q-PCR, replication kinetics, and plaque size quantification.
b
Cells infected with HSV-1 ΔUL20 display a more apoptotic phenotype than cells infected with wild-type virus, leading to release of more viral particles into the supernatant.