FIGURE 11.

Trafficking of MMP-9 vesicles is mediated by the MT motors kinesin 5B and 3B in activated macrophages. Activated (0.1 μg/ml LPS and 100 units/ml IFN-γ for 9 h) RAW 264.7 cells either untransfected or expressing kinesin 5B or 3B rigor mutants were fixed and immunostained for MMP-9 (green). Untransfected cells were stained with phalloidin to mark the cell periphery (A) (red). Alternatively, transfected cells were analyzed for either KIF5BT92N-YFP (B) or KIF3BT103N-GFP (C) (red). Scale bars, 10 μm. A quantification of the average number of MMP-9 vesicles per macrophage cell was performed (D). *, p < 0.05 compared with resting cells. Error bars, S.E.