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. 2012 Jan 23;287(11):8468–8483. doi: 10.1074/jbc.M111.290676

FIGURE 3.

FIGURE 3.

MMP-9 vesicles do not colocalize with Rab27a, Mac-1, and CD63. LPS/IFN-γ (0.1 μg/ml and 100 units/ml, respectively, for 9 h)-activated RAW 264.7 cells were analyzed for Rab27a (A), Mac-1 (B), or expressed CD63-GFP (C) (all shown in red) and MMP-9 (green), by epifluorescence. The arrows identify MMP-9 vesicles, whereas arrowheads indicate the absence of co-recruitment to MMP-9 vesicles. Scale bars, 10 μm. The percentage of MMP-9 vesicles showing co-recruitment with the indicated markers was quantified (D).