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. 2012 Jan 20;287(11):8444–8456. doi: 10.1074/jbc.M111.294124

FIGURE 7.

FIGURE 7.

A, glycerol concentration measured in the medium bathing primary murine adipocytes treated for 1 h with isoproterenol (200 nm) or norepinephrine (1 μm), showing a decrease in catecholamine-stimulated glycerol release by Angptl4−/− adipocytes (n = 6; *, p < 0.05 versus similarly treated WT cells). B, Angptl4 concentrations measured by ELISA in the epididymal WAT and plasma of mice fed ad libitum or fasted for 24 h (n = 5–6; *, p < 0.05 versus fed). C, glycerol concentration measured in the medium bathing primary murine adipocytes treated for 1 h with isoproterenol alone or plus 20 nm hANGPTL4, showing that the addition of hANGPTL4 largely rescues the impaired catecholamine-stimulated glycerol release seen in Angptl4 deficiency (n = 6; *, p < 0.05). D, cytosolic cAMP levels and glycerol release measured from WT and Angptl4−/− adipocytes treated with 20 nm hANGPTL4, showing the kinetics for each response over 60 min (n = 4–5; *, p < 0.05 versus time 0). E, dose-response relationship for the increase in cytosolic cAMP in WT and Angptl4−/− adipocytes stimulated by treatment with hANGPTL4 for 1 h (n = 8; *, p < 0.05 versus buffer alone). F, dose-response relationship for the stimulation of glycerol release into the medium by WT and Angptl4−/− adipocytes treated with hANGPTL4 for 1 h (n = 4; *, p < 0.05 versus buffer alone). Error bars, S.E.