Time dependence of neurotoxicity and CaMKII inactivation with CaMKII inhibition.
A, neuronal death (mean ± S.E., n = 7–12) normalized to control when 10 μm tat-CN21 was applied to neuronal cultures for varying lengths of time. *, p < 0.05 compared with control (one-way ANOVA, post hoc Dunnett's test). B, kinase activity (mean ± S.D., n = 3–4) in neuronal lysates subjected to an in vitro CaMKII assay in the presence of calcium/calmodulin after 10 μm tat-CN21 (or tat-CN21Ala) was applied to cortical neurons for varying lengths of time. *, p < 0.05 compared with control (one-way ANOVA, post hoc Dunnett's test).