FIGURE 3.

Neuronal apoptosis with CaMKII inhibition. Neuronal death (mean ± S.E., n = 5–10) following treatment with tat-CN21 with or without 0.5 mg/ml cycloheximide as measured by ethidium homodimer membrane permeability dye (A) or TUNEL staining (B). *, p < 0.05 compared with control; #, p < 0.05 compared with tat-CN21 alone (one-way ANOVA, post hoc Dunnett's test). C, representative image of a field of neurons treated with 10 μm tat-CN21-Fam for 24 h (top left) immunostained for cleaved caspase-3 (top right), nuclear marker Hoechst (bottom left), and a merge of all three channels (bottom right). Arrows indicate fragmented or pyknotic nuclei.