FIGURE 4.

The expression of goblet cell markers in the large intestine. A, RT-PCR of genes involved in primary differentiation from intestinal stem cells and an early goblet cell marker, Tff3. Note that the expression of Tff3 is significantly elevated in Oasis^−/− mice. B, RT-PCR of mature goblet cell markers. C, RT-PCR analysis of Spdef and Creb4. Data are mean ± S.D. n = 3. *, p < 0.05; **, p < 0.01, unpaired Student's t test. D–F, in situ hybridization of Tff3 (D), Muc2 (E), and Spdef (F) in large intestine.