TABLE 1.
Data collection and structure-solution parameters
| Parameters | Values |
|---|---|
| Crystal type | Native |
| Unit cell parameters (Å) | a = 65.39, b = 117.51, c = 199.04 |
| Resolution (Å) | 50–2.28 |
| Space group | P212121 |
| Completeness (%) | 85.6 (72.9)a |
| Rsym (%)b | 4.8 (19.8) |
| I/σ(I) | 37.1 (4.9) |
| No. of refined atoms: protein/water | 11,565/385 |
| Rfactor/Rfree (%)c | 18.7/23.7 |
| r.m.s. deviation bond length (Å) | 0.012 |
| r.m.s. deviation bond angle (degrees) | 1.116 |
| Ramachandran plot (%) | |
| Most favored region | 1293 (94.6%) |
| Additionally allowed region | 74 (5.4%) |
| Outlier region | 0 (0%) |
a Numbers in parentheses are statistics from the highest resolution shell.
b Rsym = |Iobs − Iavg|/Iobs, where Iobs is the observed individual reflection, and Iavg is the average over all symmetry equivalents.
c R factor = |Fo| − |Fc‖/|Fo|, where Fo and Fc are the observed and calculated structure factor amplitudes, respectively. Rfree was calculated using 5% of the data.