FIGURE 3.

Mpa pupylation and subsequent self-recruitment lead to disassembly of the hexameric ring. A, elution profile (recorded by absorbance at 230 nm) of mock-pupylated Mpa (light gray elution profile) (0.2 μm) and Mpa-Pup (black elution profile) (0.2 μm) as well as Mpa-Pup39S40S (gray elution profile) using a Superdex 200 size exclusion column. B, negative stain electron microscopy images of Mpa (60 nm, left ), Mpa-Pup (60 nm, middle), and Mpa-Pup39S40S (60 nm, right). White scale bars indicate 100 nm.