FIGURE 7.

ERdj4 interacts with ERAD component Derlin-1. A, COS-1 cells were transfected with pSGmERdj4 and harvested at 24 h. Cell lysates were divided evenly into four aliquots, each of which was immunoprecipitated with the indicated antibodies. IgG was used as negative control. Immunoprecipitated proteins were subjected to electrophoresis and immunoblotting with the indicated antibodies. B, immunoprecipitation from cells expressing endogenous ERdj4. 1 × 10⁸ P3U.1 cells were homogenized. The membranes were pelleted by centrifugation and lysed. The lysates were divided into equal fractions and immunoprecipitated with the indicated antibodies. IgG was used as negative control. Immunoprecipitates were subjected to electrophoresis and immunoblotting with the indicated antibodies. IP, immunoprecipitation.