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. 2012 Jan 12;287(11):8531–8540. doi: 10.1074/jbc.M111.299149

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Mutagenesis analysis of the RBBP1 CD and trimethylated lysine interaction. ¹H-¹⁵N HSQC spectra of RBBP1 CD mutants titrated with M3L are exhibited for Y583A (A) (the M3L:protein molar ratio is from 0:1 to 15:1), Y592A (the M3L:protein molar ratio is from 0:1 to 15:1) (B), Y612A (the M3L:protein molar ratio is from 0 to 15:1) (C), W615A (the M3L:protein molar ratio is from 0 to 12:1) (D), and Y619A (the M3L:protein molar ratio is from 0 to 18:1) (E). F, K_D values for binding of Y583A (upper panel) and Y592A (lower panel) to M3L.