FIGURE 5.

NEU1 regulates EGFR activation in airway ECs. A549 cells (A and C) or SAECs (E) were transfected with NEU1-targeting or control siRNAs or infected with Ad-NEU1 or Ad-Null at an m.o.i. of 100. The cells were treated with EGF or medium alone, and cell lysates were processed for phospho-EGFR (Tyr-1068) immunoblotting. To control for protein loading and transfer, blots were stripped and reprobed for β-tubulin. IB, immunoblot; IB*, immunoblot after stripping. Molecular mass in kDa is indicated on the left. Each blot is representative of two independent experiments. The densitometric analyses of the blots in A, C, and E are presented in B, D, and F, respectively. Vertical and error bars represent mean ± S.E. phospho-EGFR (pEGFR) signal normalized to β-tubulin signal in the same lane on the same blot (n = 2).