FIGURE 8.

NEU1 regulates flagellin-stimulated ERK1/2 activation in airway ECs. A549 cells (A and C) or SAECs (E) were transfected with NEU1-targeting or control siRNAs or infected with Ad-NEU1 or Ad-Null at an m.o.i. of 100. The cells were treated with P. aeruginosa flagellin or medium alone. Cell lysates were processed for phospho-ERK1/2 immunoblotting. G and H, A549 cells infected with Ad-NEU1 or Ad-Null were transfected with MUC1-targeting (H) or control (G) siRNAs, treated with flagellin or medium alone, and processed for phospho-ERK1/2 (pERK1/2) immunoblotting. To control for protein loading and transfer, blots were stripped and reprobed with anti-ERK2 antibody. IB, immunoblot; IB*, immunoblot after stripping. Molecular mass in kDa is indicated on the left. Each blot is representative of two independent experiments. The densitometric analyses of the blots in A, C, E, G, and H are presented in B, D, F, and I, respectively. Vertical and error bars represent mean ± S.E. normalized densitometry values (n = 2).