FIGURE 6.
RTV1 is expressed ubiquitously and its expression is enhanced by vernalization treatment. A, RTV1 expression in various organs as determined by RT-PCR. B, histochemical GUS staining of transgenic Arabidopsis expressing an RTV1 promoter-GUS fusion. a, cotyledon; b, shoot apical meristem; c and d, 7-day-old root; e, flower of 6-week-old plant. a and d, leaf; b and e, root; c and f, root tip. C, Northern blot analysis of RTV1 and XLG2 with (+V) or without (−V) 5 weeks of vernalization treatment. D, histochemical GUS staining of transgenic PRTV1-GUS line with (+V) or without (−V) 5 weeks of cold treatment. For GUS staining, a–c and d–f were incubated for 3 and 6 h, respectively.