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. 2012 Jan 9;287(11):8242–8253. doi: 10.1074/jbc.M111.317412

FIGURE 7.

FIGURE 7.

35S-FLAG-RTV1 plants flower early in both LD and SD, and earlier flowering is further enhanced in 35S-FLAG-XLG2 35S-RTV1-GFP plants. A, early flowering of 35S-FLAG-XLG2 35S-RTV1-GFP in LD. B, flowering times were determined as total rosette leaf number at the time of flowering under LD and SD conditions with (+V) or without (−V) 5 weeks of cold treatment. At least 12 plants of each genotype were used. Statistical significance was determined by Student's t test (*, p < 0.001 versus Col). C, all transcript levels were normalized to ACTIN2 levels using the formula, CT = CT(each gene) − CT(ACTIN2). -Fold changes are relative to results from wild type (Col). Real-time RT-PCR was performed with cDNAs from 2-week-old wild type and transgenic plants grown in SD conditions. Each bar represents an average of three independent replicate experiments. D, the expression of FLC, FLC clade (MAFs), and floral integrator genes in 35S-FLAG-XLG2 35S-RTV1-GFP plants after 5 weeks of cold treatment (+V). All results were obtained as described in C.