FIGURE 3.
Internal pH does not change unitary current amplitudes of ClC-5 in external SCN−. A and B, voltage protocols, representative ClC-5 mean currents, and variances used for non-stationary noise analysis at internal pH 6.0 (A) and pH 7.4 (B). Measurements were performed in external SCN-based solution (pH 7.4) and internal iodide-based solutions. Averaged mean steady-state currents were 2.9 ± 0.3 nA (n = 4) and 2.7 ± 0.5 nA (n = 3) at internal pH 6.0 and 7.4, respectively, with mean variances of 222 ± 9 and 237 ± 54 pA2. C and D, representative variance-current plots for the cells shown in A and B. Lines represent parabolic fits of Equation 3 to the data. E, mean unitary current amplitudes for ClC-5 at internal pH 6.0 and 7.4. The p value shows the result of a two-sample t test.