TABLE 1.
Regions targeted for mutagenesis
| Cofactor | Amino acid segment (region) | 5′-Flanking enzyme | 3′-Flanking enzyme |
|---|---|---|---|
| BB | M150–160 (A) | AflII | PpuMI |
| BB | M173–184 (B) | PpuMI | XmnI |
| BB | L168–178 (C) | NdeI | HindIII |
| BB/HB | L179–190 (D) | HindIII | BbvCI |
| HB | L215–220 (E) | AccIII | AclI |
| HB | M124–131 (F) | EcoRV | XmaI |
| HB | M144–151 (G) | XmaI | AflII |
| HB | M271–275 (H) | NcoI | BamHI |
| QB | L186–194 (I) | HindIII | BbvCI |
| QB | L212- 216 (J) | AccIII | AclI |
| QB | L220–232 (K) | AclI | DraI |
| BA | L127–131 (L) | BalI | MluI |
| BA | L146–157 (M) | MluI | NdeI |
| BA | M195–207 (N) | XmnI | BlpI |