FIGURE 6.

Interactions between domains of eIF2B expressed in human cells. A, the individual I-patch and NT domains of eIF2Bγ and ϵ and fragments of both subunits truncated as shown were GST-myc tagged at their N termini. B, GST-tagged fragments were coexpressed in HEK293 cells with the remaining eIF2B subunits. Affinity purification using glutathione beads followed by Western blot analysis showed interactions between the eIF2B fragments and remaining subunits. GST-tagged subunits are marked with an asterisk and refer to the fragment identified in the lane header.