TABLE 1.

Bacterial strains and plasmids

	Description	Ref. or source
Strains
V. fischeri
ES114	Sequenced wild-type E. scolopes light organ isolate	71
MB06859	ES114 waaL::Tnerm	This work
BK111	MB06859 harboring pVSV105	This work
BK110	MB06859 harboring pBK01	This work
MB24439	ES114 flaD::Tnerm	Footnote 3
MJM1575	ES114 harboring pVSV103	This work
E. coli
DH5α-λpir	Cloning vector	72
MJM534	CC118λpir, carries pEVS104 for E. coli to V. fischeri conjugation	73
Plasmidsa
pVSV105	pES213-based plasmid used for complementation, CamR	15
pBK01	pVSV105 containing waaL ORF and upstream region	This work
pVSV103	pES213-based plasmid containing LacZ used for marking strains, KanR	15
pEVS104	pRK2013-based plasmid, helper for E. coli to V. fischeri conjugation, KanR	73
pVSV208	pES213-based plasmid containing rfp, CamR	15

^a Abbreviations used are as follows: Kan, kanamycin; Cam, chloramphenicol.