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The effect of RasGRP4 deficiency on FcϵRI-mediated signaling. After sensitization with anti-DNP IgE, BMMCs were stimulated with DNP-HSA (100 ng/ml) for the indicated time points. Whole cell lysates were blotted with antibodies against pTyr, pErk, pp38, and pJNK. The same membranes were also reblotted with antibodies against the pan forms of MAPKs.
