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Very few T cells in mice deficient in both RasGRP1 and RasGRP4. A, the numbers of splenic T and B cells. Spleens from eight 2-month-old mice of each genotype were used in the calculation. B, expression of CD4, CD8, and B220 on splenocytes. C, the percentages of TCRβ+ cells. TCRβ expression was analyzed on CD4+ or CD8+ cells from spleens from dKO, 1KO, 4KO, and WT mice.
