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Anticoagulant activities of recombinant mMCP-6· and hTryptase-β·heparin complexes. In the depicted experiment, sodium citrate-treated human plasma (100 μl/assay) was incubated at 37 °C with heparin alone (100 μg) or with heparin bound to recombinant hTryptase-β (3.3 and 6.6 μg) or mMCP-6 (10 μg) for 60 min. The time required for thrombin to clot the treated samples relative to untreated plasma (base line) was determined using a fibrometer.
