TABLE 2.
Pattern of VDJ junctions cloned from B-splenocytes of 3′RR-deficient and WT mice
| WT mice | 3′RR-deficient mice | |
|---|---|---|
| No. of analyzed sequences | 100 | 89 |
| CDR3 length | 11.7 ± 7 | 11.4 ± 6 |
| Total N sequences (VD + DJ) | 104 | 88 |
| Total N nucleotides (VD + DJ) | 612 | 647 |
| % N sequences | 97.2 | 91.7 |
| Mean N nucleotides/sequences | 5.8 | 7.3 |
| Total P sequences (VD + DJ) | 35 | 27 |
| Total P nucleotides (VD + DJ) | 55 | 53 |
| % P sequences | 32.7 | 28.1 |
| Mean P nucleotides/sequences | 1.6 | 1.9 |
| % functional sequences | 80.0 | 89.9 |
| D usage | WT mice |
3′RR-deficient mice |
||
|---|---|---|---|---|
| % | No. | % | No. | |
| DFL16,3 | 5.00 | 5 | 3.37 | 3 |
| DST4,2 | 6.00 | 6 | 0.00 | 0 |
| DFL16,1 | 28.00 | 28 | 22.47 | 20 |
| DSP2,9 | 9.00 | 9 | 7.87 | 7 |
| DSP2,3 | 5.00 | 5 | 7.87 | 7 |
| DST4,3 | 3.00 | 3 | 2.25 | 2 |
| DFL16,2 | 2.00 | 2 | 11.24 | 10 |
| DSP2,5 | 9.00 | 9 | 10.11 | 9 |
| DSP2,2 | 16.00 | 16 | 7.87 | 7 |
| DSP2,11 | 4.00 | 4 | 12.36 | 11 |
| DSP2,8 | 2.00 | 2 | 3.37 | 3 |
| DSP2,7 | 0.00 | 0 | 2.25 | 2 |
| DST4 | 4.00 | 4 | 4.49 | 4 |
| DQ52 | 7.00 | 7 | 4.49 | 4 |