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Subcellular localization of PilQΔ136 complex. Total membranes of HB27 ΔpilQ::bleo carrying pDM12-pilQΔ25–64his were separated into IM and outer membrane (OM) fractions. The fractions were subjected to SDS-PAGE in a 3–12% polyacrylamide gradient gel followed by immunoblotting using PilQ antibodies. The PilQ complex is indicated by an arrow. The purity of IM fractions was verified by Western blot analysis using S-layer protein-specific antibodies.
