TABLE 1.
PilQ construct | Expected molecular mass (processed) | Molecular mass of detected proteins | Complex formation |
---|---|---|---|
kDa | kDa | ||
PilQ-Q | 81.1 | 81.1, 38.5 | + |
PilQΔ25–34 | 80 | 79.9, 66.2, 42.8, 32.5, 26.9 | + |
PilQΔ25–64 | 76.9 | 76.5, 66.2, 48.4, 42.8, 33.5, 32.5, 26.9 | + |
PilQΔ25–125 | 69.9 | 66.2 | + |
PilQΔ25–207 | 61 | 61.5, 33.5, 32 | − |
PilQΔ25–262 | 54.9 | − |