FIGURE 6.

Dissection of binding parameters. Standard thermodynamic quantities of Fab binding to TNF-α (Exp.) at 37 °C obtained by global analysis of ITC data (Table 1) were dissected into the contributions of rigid body binding (Bind.) and conformational change (Conf.) (see Equation 1 and the corresponding discussion). Rigid body binding parameters were calculated from the structural model (Fig. 1), whereas conformational change parameters were estimated as Conf. = Exp. − Bind.