Fig 5.

Mouse immune serum evaluation by ELISA and immuno-dot blot analysis. (A) Mouse 1 to 5 sera from bleed 3 diluted 1/20,000 in the ELISA performed with truncated MopB (10 μg/ml) or BSA (10 μg/ml) and blank, rabbit anti-mouse (1/20,000) plus anti-rabbit alkaline phosphatase, and anti-rabbit alkaline phosphatase (1/40,000) without anti-mouse antibody. (B) Immuno-dot blot screen with a 1/20,000 dilution of bleed 3 sera from mice 1 to 5 on 5% (wt/vol) milk protein control or 5 μl of E. coli or X. fastidiosa spotted directly onto nitrocellulose strips and probed with rabbit anti-mouse alkaline phosphatase and developed with NBT-BCIP.