Table 1.
Bacterial strains and plasmids used for this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Sinorhizobium strains | ||
| NGR234 | NGR234R rif-1 Nod+ Fix + | 38 |
| NGR234 pckA | NGR234R pckA::Ω Spr | 38 |
| RmP1809 | NGR234R dme-9::Ω Spr | This study |
| RmP1814 | NGR234R dmeΔ14::Ω Spr | This study |
| RmP2190 | RmP1809(pTH1582) | This study |
| RmP2191 | RmP1809(pTH2584) | This study |
| RmP2192 | RmP1814(pTH1582) | This study |
| RmP2193 | RmP1814(pTH2584) | This study |
| RmP2648 | NGR234R pckA::Ω Spr Δdme::kan | This study |
| RmP2651 | NGR234R pckA::Ω Spr Δdme | This study |
| Escherichia coli strains | ||
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 9 |
| MT616 | MT607(MM294A recA56)(pRK600) | 52 |
| Plasmids | ||
| pBAD/HisA | Expression vector; Apr | Invitrogen |
| pJQ200-mp18 | Suicide vector; Gmr 5% sucroses | 43 |
| pHP45 | Ω Spr Smr Apr | 41 |
| pKD13 | bla FRT ahp FRT PS1 PS4 oriR6K Kmr | 9 |
| pKD46 | bla PBAD gam bet exo pSC101 oriTS Ampr | 9 |
| pRK7813 | Broad-host-range cosmid vector; Mob IncP Tcr | 20 |
| pTH1582 | pJP2 with gusA from pFUS1; Tcr | 52 |
| pTH2415 | pJQ200-mp18; 1,729 bp from NGR234 dme | This study |
| pTH2429 | pTH2415 dme::Ω Spr Smr at 418 bp | This study |
| pTH2457 | pJQ200-mp18; bp −471 to 87 of NGR234 dme | This study |
| pTH2462 | pJQ200-mp18; bp −471 to 87 and 587 to 1186 of NGR234 dme | This study |
| pTH2467 | pTH2462::Ω Spr Smr (between bp 87 and 587) | This study |
| pTH2505 | FLP gene in pRK7813 | Unpublished |
| pTH2530 | pJQ200-mp18; bp −471 to 87 and 1823 to 920 of NGR234 dme | This study |
| pTH2532 | pTH2530 Δdme::FRT Kmr Nmr from pKD13 | This study |
| pTH2584 | pTH1582; sequence from bp −284 and complete NGR234 dme gene | This study |
| pTH2754 | azc3656 with 5′ NheI site and 3′ HindIII site inserted into NheI and HindIII sites of pBAD/HisA | This study |
| pTH2755 | azc0119 with 5′ NheI site and 3′ HindIII site inserted into the NheI and HindIII sites of pBAD/HisA | This study |