. 2012 Apr;78(8):2803–2812. doi: 10.1128/AEM.06412-11

Table 2.

Enzyme activities of Sinorhizobium sp. NGR234 wild-type and dme mutant bacteroids and free-living cells

Host plant^a	Enzyme	Sp act^c
Host plant^a	Enzyme	NGR234 wild type	NGR234 dme-9::Ω Sp^r	NGR234 dmeΔ14::Ω Sp^r
Cajanus cajan	DME	42 ± 1	10 ± 1	10 ± 2
	TME	26 ± 3	27 ± 3	20 ± 4
	PCK	99 ± 9	95 ± 4	86 ± 2
	MDH	2,919 ± 157	3,394 ± 183	3,466 ± 463
Lablab purpureus	DME	46 ± 3	7 ± 3	7 ± 1
	TME	53 ± 4	38 ± 3	42 ± 5
	PCK	75 ± 6	52 ± 1	54 ± 2
	MDH	2,675 ± 89	3,137 ± 94	3,223 ± 61
Leucaena leucocephala	DME	49 ± 2	24 ± 2	22 ± 2
	TME	15 ± 2	11 ± 3	9 ± 2
	PCK	47 ± 2	41 ± 3	38 ± 4
	MDH	1,648 ± 129	1,818 ± 109	1,577 ± 117
Macroptilium atropurpureum	DME	67 ± 4	21 ± 4	21 ± 1
	TME	45 ± 5	39 ± 4	33 ± 4
	PCK	182 ± 10	160 ± 1	156 ± 3
	MDH	2,592 ± 74	3,344 ± 204	3,912 ± 91
Vigna unguiculata	DME	74 ± 2	23 ± 2	21 ± 1
	TME	50 ± 5	35 ± 4	35 ± 1
	PCK	113 ± 4	100 ± 1	96 ± 3
	MDH	1,736 ± 211	1,613 ± 74	2,301 ± 193
Free-living cells^b	DME	103 ± 13	51 ± 3	44 ± 7
	TME	80 ± 4	67 ± 6	61 ± 8
	MDH	995 ± 50	1,395 ± 153	1,191 ± 49

Bacteroids were isolated from nodules of 6-week-old Cajanus cajan, 10-week-old Lablab purpureus, 6-week-old Macroptilium atropurpureum, and 6-week-old Vigna unguiculata plants.

Free-living cells of wild-type and dme mutant strains were grown in TY.

Values are means ± standard errors for triplicate samples. For DME (NAD-dependent malic enzyme), specific activity is shown as nanomoles of pyruvate formed/minute/mg protein; for TME (NADP-dependent malic enzyme), specific activity is shown as nanomoles of pyruvate formed/minute/mg protein; for PCK (phosphoenolpyruvate carboxykinase), specific activity is shown as nanomoles of NADH oxidized/minute/mg protein; and for MDH (malate dehydrogenase), specific activity is shown as nanomoles of NADH formed/minute/mg protein. Enzyme activities in S. meliloti bacteroids were measured as 184 ± 7 nmol/min/mg protein for DME, 5,078 ± 109 nmol/min/mg protein for MDH, and 8 ± 2 nmol/min/mg protein for PCK.