Fig 1.

Emission of methane. (A) Living earthworms and gut content; (B) substrates. Results marked with an asterisk are from the sampling in March 2011 and a 5-h incubation. Results not marked with an asterisk are from the sampling in September 2011 and a 6-h incubation. Filled squares indicate mean values, and lines indicate lowest and highest values. Codes: S, substrate; first number after S, substrate on which worms were raised and maintained (e.g., S1 is substrate 1); second S and accompanying number, the substrate to which worms were transferred and maintained for 60 h prior to assay (e.g., S1/S2 indicates that worms raised on substrate 1 were transferred to and maintained on substrate 2). H₂ indicates that the headspace contained 1.5% H₂ and 0.4% CO₂; BES indicates that assays were supplemented with BES; S0 indicates that worms were received in diapause without gut content; S01 indicates that worms were received in diapause without gut content and incubated on substrate 1 for 60 h.