Emission of methane by representative specimens of E. eugeniae and substrate 1. Symbols: squares, E. eugeniae raised and maintained on substrate 1; circles, E. eugeniae raised on substrate 1 and transferred to substrate 2 for 60 h prior to assay; diamonds, E. eugeniae raised on substrate 1 and transferred to substrate 4 for 60 h prior to assay; triangles, substrate 1; empty symbols, headspace was air; filled symbols, headspace was air supplemented with 1.5% H2 and 0.4% CO2.