Table 2.
Relative-rate tests for the 16S rRNA and groEL gene sequences of the lineages of I. sabuleti, A. longiceps, B. numenius, O. depressus, and O. punctipennis endosymbionts and Escherichia coli and Pectobacterium carotovorum as free-living relatives, as well as Vibrio cholerae as an outgroup
| Host and gene | Organism(s) (accession no.) in: |
K1a | K2b | K1 − K2 (mean ± SD) | Rate ratio (K1/K2) | P valuec | ||
|---|---|---|---|---|---|---|---|---|
| Lineage 1 | Lineage 2 | Outgroup | ||||||
| Ischnodemus | ||||||||
| 16S rRNA | Endosymbiont of I. sabuleti (HE586115) | E. coli (J01695), P. carotovorum (AF373185) | V. cholerae (X74694) | 0.125 | 0.098 | 0.027 ± 0.0090 | 1.27 | 0.003 |
| groEL | Endosymbiont of I. sabuleti (HE586269) | E. coli (AY569651), P. carotovorum (CP001657) | V. cholerae (CP001235) | 0.174 | 0.139 | 0.036 ± 0.0212 | 1.26 | 0.093 |
| Arocatus | ||||||||
| 16S rRNA | Endosymbiont of A. longiceps (HE586116) | E. coli (J01695), P. carotovorum (AF373185) | V. cholerae (X74694) | 0.115 | 0.098 | 0.017 ± 0.0068 | 1.17 | 0.011 |
| groEL | Endosymbiont of A. longiceps (HE586268) | E. coli (AY569651), P. carotovorum (CP001657) | V. cholerae (CP001235) | 0.194 | 0.159 | 0.035 ± 0.0159 | 1.22 | 0.027 |
| Belonochilus | ||||||||
| 16S rRNA | Endosymbiont of B. numenius (HE586117) | E. coli (J01695), P. carotovorum (AF373185) | V. cholerae (X74694) | 0.167 | 0.096 | 0.071 ± 0.0088 | 1.74 | 1e−07 |
| groEL | Endosymbiont of B. numenius (HE586267) | E. coli (AY569651), P. carotovorum (CP001657) | V. cholerae (CP001235) | 0.178 | 0.159 | 0.019 ± 0.0149 | 1.12 | 0.197 |
| Orsillus | ||||||||
| 16S rRNA | Endosymbiont of O. depressus (HE586114) | E. coli (J01695), P. carotovorum (AF373185) | V. cholerae (X74694) | 0.143 | 0.099 | 0.044 ± 0.0095 | 1.44 | 5e−06 |
| groEL | Endosymbiont of O. depressus (HE586266) | E. coli (AY569651), P. carotovorum (CP001657) | V. cholerae (CP001235) | 0.210 | 0.159 | 0.051 ± 0.0194 | 1.32 | 0.008 |
| Ortholomus | ||||||||
| 16S rRNA | Endosymbiont of O. punctipennis (HE586113) | E. coli (J01695), P. carotovorum (AF373185) | V. cholerae (X74694) | 0.148 | 0.099 | 0.049 ± 0.0097 | 1.49 | 1e−06 |
| groEL | Endosymbiont of O. punctipennis (HE586265) | E. coli (AY569651), P. carotovorum (CP001657) | V. cholerae (CP001235) | 0.201 | 0.159 | 0.042 ± 0.0190 | 1.27 | 0.026 |
a
Estimated mean distance between lineage 1 and the last common ancestor of lineages 1 and 2.
b
Estimated mean distance between lineage 2 and the last common ancestor of lineages 1 and 2.
c
P values were generated using the program RRTree.