Fig 1.

Comparative CLSs and stress responses of strains. (A) CLS analysis of industrial yeast strains in SC medium. The assays were performed by making serial dilutions, plating an aliquot, and counting colonies. Counts of CFU/ml were done in relation to the values obtained from day 3 postinoculation and were considered 100% survival. The stress tolerance of strains EC1118 and CSM grown exponentially (B) or to the stationary phase (C) in YPD medium was also determined. Stress was applied, and serial dilutions were made and plated. Cell viability values were determined relative to viability before the stress conditions. Experiments were done in triplicate, and means and standard deviations are shown. *, P < 0.05; **, P < 0.01 (unpaired, two-tailed t test). EtOH, ethanol.