Fig 4.

Rhodanese Rdl2p plays a role in oxidative stress response, ROS levels, and life span. (A) Viability after oxidative stress caused by H₂O₂ of stationary-phase cultures of rdl1Δ, rdl2Δ and rdl1Δrdl2Δ mutants and their parental strain C9 in YPD medium. (B) Synthetic grape juice fermentation by the rdl2Δ mutant and C9. Cell viability, measured as CFU/ml, and total sugars are indicated over time. (C) ROS production measured during fermentation described in panel B. Experiments were done in triplicate, and means and standard deviations are provided. *, P < 0.05; **, P < 0.01; ***, P < 0.005 (unpaired, two-tailed t test). wt, wild type.