Fig 1.

Differential chemokine receptor expression on CD25^high, CD25^low, and CD25⁻ CD4⁺ T cells. Freshly isolated human PBMCs were stained extracellularly for CD4, CD25, CCR3, CCR4, CXCR3, CCR6, and CD127 and intracellularly for FOXP3 markers and analyzed by flow cytometry. (A) CD4⁺ T lymphocytes were divided according to CD25 expression. (B) Gated CD25^high (nTreg), CD25^low (effector Th [Teff]), and CD25⁻ (resting) CD4⁺ T cells were analyzed for the expression of chemokine receptors and Treg markers. Bars represent the mean and standard deviation of MFI obtained in five independent experiments performed with different blood donors. Statistical differences between CD25^high and CD25⁻ cells were evaluated by t test. *, P < 0.05; **, P < 0.01.