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Strategy used to construct the Cyp 4a14 pNTK targeting vector and for genotype analysis: shown are a partial restriction analysis and exon/intron distribution of the Cyp 4a14 gene and a pNTK targeting vector in which exons 10 and 11 are replaced by a neomycin resistance gene. Included is a Southern analysis of a HindIII digest of tail DNA by using the indicated 1.8-kb DNA probe.
