Table 1.

Plasma androgen levels in Cyp 4a14 (+/+) and (−/−) male mice

Source of plasma		DHT	TST
Control	(+/+)	0.44  ± 0.05	1.16  ± 0.1
	(−/−)	1.02  ± 0.1*	2.06  ± 0.3*
CST/PL	(+/+)	≤0.05†	≤0.20†
	(−/−)	≤0.05†	≤0.20†
CST/DHT	(+/+)	2.64  ± 0.3**	0.89  ± 0.1
	(−/−)	2.01  ± 0.1**	0.82  ± 0.1

Fourteen-week-old male Cyp 4a14 (+/+) and (−/−) mice were castrated and, 10 days later, implanted with either placebo (CST/PL) or DHT (CST/DHT)-releasing pellets (21-day pellets, 5 mg DHT/day; Innovative Research of America, Sarasota, FL). Ten days after implantation, plasma samples were analyzed for TST and DHT levels by RIA using commercially available kits. Values (in nanograms/milliliters of plasma) are the mean ± SE of at least 31 different animals for control Cyp 4a14 (+/+) and (−/−) mice and of at least 10 different animals for the rest. Significantly different from control wild type:  

^*P ≤ 0.0009 and P ≤ 0.005 for DHT and TST, respectively. Significantly different from control wild-type and knockout mice, respectively:  

^**P ≤ 2 × 10⁻⁴ and P ≤ 1 × 10⁻³ for castrated DHT-treated (+/+) and (−/−) mice.  

^†, below assay detection limit.