Table 2.
The microsomal Arachidonic acid ω-hydroxylase of mouse kidney microsomes
| Microsomes | ω-Hydroxylase rate | (20-HETE) % of total | |
|---|---|---|---|
| Males | |||
| Control | (+/+) | 38 ± 2 | 86 |
| Control | (−/−) | 85 ± 9* | 84 |
| CST/PL† | (+/+) | <0.2 | — |
| CST/PL† | (−/−) | <0.2 | — |
| CST/DHT | (+/+) | 228 ± 35** | 82 |
| CST/DHT | (−/−) | 225 ± 23 | 82 |
| Females | |||
| Placebo | (+/+) | <0.2 | — |
| Placebo† | (−/−) | <0.2 | — |
| DHT | (+/+) | 140 ± 3 | 76 |
| DHT | (−/−) | 164 ± 3 | 77 |
Fourteen-week-old castrated male and female Cyp 4a14 (+/+) and (−/−) mice were implanted with either placebo (CST/PL) or DHT-releasing pellets (CST/DHT) (Table 1). Ten days later, kidney microsomes were incubated with AA, as described in Methods. Rates, in picomoles of product formed per minute per milligram of microsomal protein, were calculated from the corresponding time courses of product formation. Values are averages ± SE calculated from at least six (males) or three (females) different experiments. Significantly different from control wild type:
, P ≤ 1 × 10−4 and P ≤ 5 × 10−4 for total AA metabolism and 20-HETE formation, respectively. Significantly different from control wild-type and knockout mice, respectively:
, P ≤ 7 × 10−4 and P ≤ 9 × 10−4 for castrated DHT-treated (+/+) and (−/−) mice.
Total reaction rates below detection limits.