Table 4.
Parameters of MRM Experiment for seven candidate proteins.
| Protein name | Q1a | Q3b | Sequencec | Fragmentd | Chargee | CEf |
|---|---|---|---|---|---|---|
| Transferrin | 482.8 | 682.4 | APNHAVVTR | y6 | 2+ | 26 |
| 315.2 | 558.3 | AVGNLR | y5 | 2+ | 19 | |
| 315.2 | 459.3 | AVGNLR | y4 | 2+ | 19 | |
| Ceruloplasmin | 686.4 | 1080.0 | GAYPLSIEPIGVR | y10 | 2+ | 35 |
| 686.39 | 870.5 | GAYPLSIEPIGVR | y8 | 2+ | 35 | |
| Alpha-1-antitrypsin | 555.81 | 997.5 | LSITGTYDLK | y9 | 2+ | 29 |
| 555.81 | 797.4 | LSITGTYDLK | y7 | 2+ | 29 | |
| 393.2 | 587.3 | VVNPTQK | y5 | 2+ | 22 | |
| 393.2 | 473.3 | VVNPTQK | y5 | 2+ | 22 | |
| Haptoglobin precursor | 729.8 | 1084.5 | NLFLNHSENATAK | y10 | 2+ | 37 |
| 352.2 | 517.3 | VSVNER | y4 | 2+ | 20 | |
| Vitamin D-binding protein | 400.2 | 700.4 | VLEPTLK | y6 | 2+ | 26 |
| 400.2 | 587.3 | VLEPTLK | y5 | 2+ | 26 | |
| Alpha-1-acid glycoprotein 1 | 556.8 | 811.4 | SDVVYTDWK | y6 | 2+ | 29 |
| 580.8 | 974.5 | WFYIASAFR | y8 | 2+ | 31 | |
| 580.8 | 827.4 | WFYIASAFR | y7 | 2+ | 31 | |
| Prostate stem cell antigen | 501.0 | 830.5 | AVGLLTVISK | y8 | 2+ | 30 |
| 501.0 | 660.4 | AVGLLTVISK | y6 | 2+ | 30 |
a-bQ1 and Q3 (m/z) represent the Q1 and Q3 transitions for proteotypic peptide, respectively. cSequence represents the sequence of proteotypic peptide for target protein. dFragment type indicates the ion type of the Q3 transition. eCharge represents the charge state of precursor ion. fCE represents collision energy.