Fig. 4.

Stimulation with FasL and TNF-α induced apoptosis of cultured tubular epithelial cells. Apoptotic cells were detected as a sub G1 population by FACS analysis following propidium iodide staining. Representative data of unstimulated cells (ai) and combined stimulation with FasL (500 U/ml) and TNF-α (500 ng/ml) (aii). Quantification of the levels of apoptosis is shown in b following stimulation of cultured cells for 24 h with FasL and/or TNF-α at the indicated concentrations. Values are mean ± SEM. * p < 0.05 compared with unstimulated control cells.