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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Neurobiol Aging. 2011 Dec 27;33(6):1125.e9–1125.e18. doi: 10.1016/j.neurobiolaging.2011.11.023

Figure 1. CAST overexpression decreases APP metabolite levels in depositing APP23/CAST mouse brain. (A–B).

Figure 1

Brain sections of depositing 13-month-old APP23/CAST and APP23 mice were used to visualize and quantify β-amyloid plaque area by Thioflavin S staining. (C) Formic acid-extractable human Aβ40 and Aβ42 levels were measured by sandwich ELISA. (D) Analysis of Aβ38, Aβ40 and Aβ42 using Western blots as previously described by Klafki et al. (Klafki, et al., 1996) based upon migration of Aβ standards. (E) MALDI-TOF mass spectrometry of brain homogenates immunoprecipitated simultaneously with 6E10 and 4G8 antibodies. (F) Neprilysin and IDE levels are shown by Western blot analysis of brain homogenates. (G) By Western blot, total proteins probed for APP and CTFs; soluble brain extracts lacking membrane-associated proteins probed for sAPP total, sAPPα and sAPPβ, as indicated (Morales-Corraliza, et al., 2009). (H) Graphic representation of the analysis of the band density of the blots of APP, CTFs and sAPP total. *p<0.05; ** p<0.01; ***p<0.001.