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. 2012 Mar 7;32(10):3516–3528. doi: 10.1523/JNEUROSCI.3905-11.2012

Figure 4.

Figure 4.

Dexamethasone and prednisolone potentiate support cell proliferation. A, In fish co-treated with 5 μm dexamethasone (Dex) or prednisolone (Pred) and BrdU without prior neomycin treatment (No Neo), there is an increase in the number of GFP-positive cells (p < 0.0001) and a comparable increase in the number of GFP+/BrdU+ hair cells (p < 0.01), suggesting that the increase in hair cells arose from support cell proliferation and differentiation of new hair cells. B, In larvae treated with 400 μm neomycin (Neo) and then allowed to recover in 5 μm dexamethasone or prednisolone for 48 h in the presence of BrdU, there was a significant increase of GFP-labeled hair cells (p < 0.0001) and a comparable increase in BrdU-labeled hair cells (p < 0.0001) for dexamethasone and prednisolone-treated groups compared with DMSO vehicle controls. Means of total hair cells in seven neuromasts per fish (n = 10 fish/group) are presented. Error bars indicate 1 ± SD.