Figure 6.

Dose–response functions for six confirmed regeneration inhibitors. Fish were acutely exposed to 400 μm neomycin (Neo) in embryo media for 1 h (or mock-treated; No Neo), and then incubated in 0, 0.1, 1, 5, 10, 50, and 100 μm of the noted inhibitor drug in EM for 48 h during the regeneration period. Hair cell regeneration was assessed using the DASPEI scoring system. For each fish (n = 10–12 fish/group), 10 neuromasts were scored, and total DASPEI score was recorded. Means ± 1 SD are shown. All six inhibitor drugs show significant suppression of hair cell regeneration over samples that received no neomycin treatment (p < 0.01). For both artesunate and fenoprofen, significant drug toxicity was noted in the mock-treated controls at high concentrations.