Figure 9.

Flubendazole and topotecan arrest, but do not eliminate, hair cell regeneration. Fish were treated with neomycin, exposed to flubendazole or topotecan for 24 h (or mock-treated with DMSO; cross-hatched area on abscissa), and then placed in fresh embryo media for an additional 72 h. A, Hair cell numbers in fish treated with flubendazole remain low while flubendazole is present (through 24 h). When flubendazole was removed, hair cell regeneration resumes, and by 96 h, hair cell numbers in flubendazole-treated and DMSO-treated controls are not significantly different. B, Similar suppression of hair cell regeneration is seen in fish treated with topotecan for 24 h. However, when the inhibitor drug was removed, hair cell regeneration was greatly attenuated. Hair cells were counted in seven neuromasts per fish (n = 10 fish per group). Error bars indicate ±1 SD.