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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Mol Cancer Ther. 2010 Sep 3;9(11):3065–3073. doi: 10.1158/1535-7163.MCT-10-0623

Figure 1. Design of an in vivo FRET assay to monitor Bcr-Abl activity in CML.

Figure 1

The assay is based on the in vivo phosphorylation of the Crk II-derived Picchu biosensor on Tyr(Y)221 by Bcr-Abl. Schematic representation shows that Picchu Y221 phosphorylation by Bcr-Abl induces an intramolecular association between the SH2 domain and pTyr221, bringing the N-terminal and C-terminal ends of the molecule together in a conformation change and yielding an energy transfer from CFP to YFP.