Table 1.
Concentration of flavonoid glycosides and naphthodianthrones in dissected Tenthredo zonula immature stages
| Sawfly stage/Plant | Qtt | Organ | Rn | He | In | Qn | Pn | Hn |
|---|---|---|---|---|---|---|---|---|
| Larva/ Hypericum perforatum, cut |
39 μl | Hem. Hem.a |
– | – | – | – | – | 0.01±0.15 [TR] |
| 4 | Dig. tr. | – | – | – | – | 0.02±0.14 | – | |
| 3 | Sal. gl. | – | – | – | – | – | – | |
| 4 | Misc. org. | – | – | – | 54.72±13.61 | TR | 0.01±0.17 | |
| Larva/ H. perforatum, cut |
44 μl | Hem. Hem.a |
– | – | – | – | TR [TR] |
0.02±0.19 [TR] |
| 5 | Dig. tr. | – | – | – | 4.10±0.69 | – | – | |
| 2 | Sal. gl. | 2.07±0.45 | – | – | – | TR | – | |
| 5 | Misc. org. | – | – | – | – | – | – | |
| Larva/ H. perforatum, cut |
40 μl | Hem. Hem.a |
– | – | – | OV [OV] |
– | – |
| 5 | Dig. tr. | – | TR | 1.84±0.67 | – | TR | – | |
| 4 | Sal. gl. | – | – | – | – | – | – | |
| 5 | Misc. org. | – | – | – | – | TR | – | |
| Larva/ H. perforatum, cut |
35 μl | Hem. Hem.a |
– | 3.25±1.71 [0.20±0.21] |
– | OV [OV] |
– | – |
| 4 | Dig. tr. | – | 1.48±0.47 | – | – | 0.25±0.17 | – | |
| 3 | Sal. gl. | 0.96±0.33 | – | 10.46±4.23 | – | – | – | |
| 4 | Misc. org. | – | – | 7.96±1.19 | 62.48±16.72 | TR | – | |
| Larva/ H. perforatum, potted |
46 μl | Hem. Hem.a |
– | – | – | OV [OV] |
– | – |
| 6 | Dig. tr. | – | 2.60±0.69 | 6.20±1.09 | – | 0.02±0.19 | ||
| 2.5 | Sal. gl. | – | – | – | – | – | – | |
| 6 | Misc. org. | TR | – | – | – | – | – | |
| Larva/ H. perforatum, potted |
40 μl | Hem. Hem.a |
– | – | – | – | – | – |
| 7 | Dig. tr. | – | – | 0.86±0.31 | – | 0.06±0.22 | ||
| 3.5 | Sal. gl. | – | – | – | – | – | – | |
| 7 | Misc. org. | 0.86±0.24 | – | – | – | – | – | |
| Prepupa/ H. perforatum, cut |
48 μl | Hem. Hem.a |
4.97±1.17 [0.15±0.23] |
8.08±1.68 [0.36±0.19] |
10.03±2.12 [0.45±0.31] |
– | – | 0.04±0.16 [TR] |
| 6 | Sal. gl. | 41.19±11.63 | – | 32.33±12.06 | – | – | 0.06±0.13 | |
| 6 | Misc. org. | 4.59±1.14 | 2.38±0.96 | – | – | TR | 0.01±0.09 | |
| Prepupa/ H. hirsutum, potted |
24 μl | Hem. Hem.a |
– | – | – | – | – | – |
| 4 | Sal. gl. | – | – | – | – | – | – | |
| 4 | Misc. org. | – | – | – | – | – | – | |
| Excrement | 0.11±<0.01 | 0.12±0.01 |
For eight batches of 4–7 individuals, hemolymph (Hem.) was collected prior to dissection of digestive tract (Dig. tr.), salivary glands (Sal. gl.), or remaining, miscellaneous organs (Misc. org.). The quantity in μl, or number of organs, is indicated in the column headed Qtt. Data are given as mean±SD of three technical replicates, in μg/individual. Values of hemolymph are also given in μmol/g FW (Hem.a, in brackets). If the compound was present at <0.005 of the given concentration unit, it is recorded as TR, and if it was not detected it is indicated with a dash (–). OV indicates overlapping peaks, which made quantification impossible. The compounds are: rutin (Rn), hyperoside (He), isoquercitrin (In), quercitrin (Qn), pseudohypericin (Pn), and hypericin (Hn)