Fig. 6.

(a) Phase contrast and fluorescence images of ES cell-derived aggregates on day 9 of culture in 300 µm wells. Green fluorescence indicates live, red fluorescence indicates dead cells. (b) Typical retrieved cell aggregates in phase contrast and fluorescence images: ES cells aggregates and (c) HepG2 cells, both formed in 150 µm wells. Scale bars are 150 µm. (d) Retrieval rate of ES cell and HepG2 cell aggregates from 150 and 300 µm wells after a 9 day culture. The error bars are standard deviation, with n = 3.