Figure 4.

MD leads to an increase in mEPSC amplitude and frequency. A, B, Left, Representative whole-cell voltage-clamp mEPSCs recorded from four relay neurons in contralateral and ipsilateral, deprived (MD, black) and nondeprived (open, gray) eye regions (CDE, COE, IDE, and IOE) of a monocularly deprived (7 d) mouse at P14. The accompanying diagrams depict deprived and nondeprived targeted regions. The mEPSCs were recorded in TTX (1 μm) and BIC (25 μm) at a holding potential of −70 mV. Highlighted sections are expanded in time below each trace. Middle, Average mEPSCs recorded from CDE, COE, IDE, and IOE regions. Amplitude was increased in CDE regions (black top trace). Right, Frequency histogram plots of mEPSC amplitude (left) and frequency (right). Deprivation shifted the distribution of mEPSCs in CDE regions toward larger amplitude and smaller interevent interval values (top). C, Group-averaged mEPSC amplitude (left) and frequency (right) after MD. Error bars depict SE. Dashed lines show the average amplitude and frequency of events recorded in age-matched normally reared mice (Fig. 3). Shaded area is ±SE. MD significantly increased the mEPSC amplitude (left, *p < 0.05) and frequency (right, *p < 0.01 for all comparisons) of mEPSCs in CDE regions. CDE, n = 28 cells; IOE, n = 12 cells; COE, n = 22 cells; IDE, n = 15 cells. D, Histograms plot the duration (time from maximal to 10% of peak amplitude, bin width = 0.5 ms) of mEPSCs for CDE and IOE (top) and COE and IDE regions (bottom).