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Effect of pH on the TM and hyperchromicity of unmodified DNA and 5-HO-dC (X) substituted DNA at 100 mM NaCl in 10 mM sodium phosphate buffer.
| pH | GAGAGCGCTCT TM(°C) | Hyperchromicity (%) | GAGAGCGCTXTC TM (°C) | Hyperchromicity (%) |
|---|---|---|---|---|
| 5.5 | 52.7 | 13.4 | 22.1 | 11.9 |
| 6.4 | 55.1 | 12.5 | 27.4 | 9.2 |
| 7.0 | 55.3 | 12.9 | 28.2 | 10.2 |
| 8.5 | 54.7 | 14.1 | 25.8 | 13.3 |
